Research Assistant Technician 4

Job Summary

• This position is required to support day to day infrastructure operation and user training in the High Resolution Macromolecular EM Facility at UBC;
• to assist with operation of a variety of instrumentation required for high resolution single particle cryo electron microscopy (cryoEM) sample preparation, data collection and data analysis;

to provide support to users in sample grid preparation as well as room

temperature and cryoEM imaging under oversight of the HRMEM Operational and

Infrastructure Lab Managers.

Organizational Status

The incumbent will report directly to the Lab manager and the PI, Dr. Natalie Strynadka.

Work Performed

• Oversee, operate, maintain, train users on a 120 keV Talos TEM instrument in both room temperature and cryo modes (including the use of a side cryo holder).
• Responsible for handling and clipping cryoEM grids in preparation for imaging, as well as handling of cryoEM grids for transfer to multi-sample autoloader capsules.
• Screen samples on a 200 keV Glacios cryo TEM instrument.
• Oversee, maintain, train users on a VitroBot and other vitrification equipment.
• Train users on data collection software and analysis for single particle macromolecular negative stain and cryoEM applications.
• Work with users to troubleshoot problems and answer queries.
• Oversee and perform logistical tasks including session scheduling, placing orders, handling liquid nitrogen, and tracking usage.

Consequence of Error / Judgement

Poor decisions and / or coordination could delay the timely completion of projects or may result in the loss of funding.

Supervision Received

Work under limited supervision and sets own priorities based on work load. Much of the work is done independently, however, work is reviewed against task objectives and conformity to standards.

Supervision Given

Provides guidance and direction to faculty, staff and graduate / undergraduate students.

Minimum Qualifications

Completion of a university degree in a relevant discipline or technical program and a minimum four years of related experience or an equivalent combination of education and experience.

Some positions may require a graduate degree.

• Willingness to respect diverse perspectives, including perspectives in conflict with one’s own
• Demonstrates a commitment to enhancing one’s own awareness, knowledge, and skills related to equity, diversity, and inclusion

Preferred Qualifications

Undergraduate degree in a relevant discipline, or graduation from a technical college or institute is required. A MSc in a relevant discipline is preferred.

Prior expertise of transmission electron microscopy would be desirable.

Candidates should be able to :

• communicate effectively verbally and in writing, including accurate record keeping.
• work in a team environment.
• work independently with limited supervision. -
• have attention to detail and a technical mindset
• use lateral thinking skills to resolve problems
• deal with a diversity of people in a calm, courteous, and effective manner.
9 days ago