Research Technician III

Company Description

UHN is Canada’s #1 hospital and the world’s #1 publicly funded hospital. With 10 sites and more than 44,000 TeamUHN members, UHN consists of Toronto General Hospital, Toronto Western Hospital, Princess Margaret Cancer Centre, Toronto Rehabilitation Institute, The Michener Institute of Education and West Park Healthcare Centre. As Canada's top research hospital, the scope of biomedical research and complexity of cases at UHN have made it a national and international source for discovery, education and patient care. UHN has the largest hospital-based research program in Canada, with major research in neurosciences, cardiology, transplantation, oncology, surgical innovation, infectious diseases, genomic medicine and rehabilitation medicine. UHN is a research hospital affiliated with the University of Toronto.

UHN’s vision is to build A Healthier World and it’s only because of the talented and dedicated people who work here that we are continually bringing that vision closer to reality.

Job Description

Union: Non-Union
Number of Vacancies: 1
New or Replacement Position: New
Site: Princess Margaret Cancer Centre
Department: Research
Reports to: Principal Investigator
Salary Range: $73,069 - $91,347 Per Year
Hours: Hours Per Week
Shifts: Day shifts; Monday - Friday
Status: Temporary Full-Time (1 year contract)
Closing Date: August 14, 2026

Position Summary:
Research Technician III serves as wet-lab lead for recombinant protein production and ensuring the technical alignment of the protein production team. This is a senior bench-focused role responsible for the daily execution of experiments, material logistics, and data integrity.

Duties

• Protein ProductionFunction as the lead for wet-lab insect cell protein production to meet project milestones.Collect and record experimental data, ensuring accuracy and rigorous quality control assessments on production batches.
• Laboratory ManagementSupport global logistics for protein distribution, managing the team’s inventory and coordinating documentation for all incoming and outgoing shipmentsPrepare, assemble, and set up equipment, reagents, and supplies for protein production workflows.Proactively manage the lifecycle of laboratory consumables, identifying procurement needs and stocking requirements before they impact production timelines.Organize routine equipment checks, CIP procedures, and notify the lab manager of any issues.
• Data & RecordsEnter and maintain accurate data in laboratory databases and shared records.Prepare and present summaries of production runs and QC and enforce traceability standards for the labeling and storage of biological samplesServe as the primary point of contact for the documentation and logistics for material transfers
• Health & SafetyFollow established laboratory safety guidelines at all biological materials, chemicals, and equipment in compliance with safety protocols.Contribute to maintaining a safe and orderly work environment.

Qualifications

• At minimum, completion of a Bachelor’s degree program, or recognized equivalent in health or science related discipline required
• At minimum, 6 years of relevant independent, hands-on experience producing recombinant proteins in insect cells5 years experience working in a structural biology laboratory an asset
• Experience with secreted and intracellular insect cell protein expression using BEVS and Sf9
• At least 5 years experience purifying recombinant proteins (affinity, IEX, SEC…) and QC using Mass Spectrometry, DSF and/or related methods an asset
• Experience in high-throughput protein production
• Experience with mammalian systems considered a plus
• Proven ability to optimize and scale parallel protein expression and purification
• Proficiency with automated FPLC systems
• Ability to follow established protocols with accuracy and attention to detail.
• Strong record-keeping and data entry skills.
• Demonstrated commitment to safety practices.
• Ability to work collaboratively in a team environment.

Additional Information

Why join UHN?

In addition to working alongside some of the most talented and inspiring healthcare professionals and researchers in the world, UHN offers a wide range of benefits, programs and perks. It is the comprehensiveness of these offerings that makes it a differentiating factor, allowing you to find value where it matters most to you, now and throughout your career at UHN.

• Competitive offer packages
• Government organization and a member of the Healthcare of Ontario Pension Plan (HOOPP )
• Close access to Transit and UHN shuttle service
• A flexible work environment
• Opportunities for development and promotions within a large organization
• Additional perks (multiple corporate discounts including: travel, restaurants, parking, phone plans, auto insurance discounts, on-site gyms, etc.)

Current UHN employees must have successfully completed their probationary period, have a good employee record along with satisfactory attendance in accordance with UHN's attendance management program, to be eligible for consideration.

All applications must be submitted before the posting close date.

UHN uses email to communicate with selected candidates. Please ensure you check your email regularly. At University Health Network (UHN), artificial intelligence technologies may be used to assist in the screening, assessment, and selection of candidates for this position.

Please be advised that a Criminal Record Check may be required of the successful candidate. Should it be determined that any information provided by a candidate be misleading, inaccurate or incorrect, UHN reserves the right to discontinue with the consideration of their application.

UHN is an equal opportunity employer committed to an inclusive recruitment process and workplace. Requests for accommodation can be made at any stage of the recruitment process. Applicants need to make their requirements known.

We thank all applicants for their interest, however, only those selected for further consideration will be contacted.